Human telencephalic organoid development in the presence and absence of exogenous extracellular matrix

Long-term culture of human telencephalic organoids cultured in the presence or absence of exogenous ECM. To evaluate how ECM supplementation influences organoid development, we supplied exogenous ECM in the form of Matrigel at the beginning of neuroepithelialisation (day 10, D10), either as a solid droplet that provides long-term exposure to a polymerized network of ECM proteins (droplet embedding - condition "drop" in the metadata table), or transiently dissolved in the culture medium (concentration of 2%V/V ) from D10 to D13 (liquid embedding - "liq"). These protocols were compared to one without exposure to exogenous ECM ("no"). Organoids were processed at 120 days of culture, when mature cortical tissue is present. scRNA-Seq; Human telencephalic organoids; 3 replicates per condition of exogenous ECM supplementation (in the metadata file: orgid 1 to 9; condition "drop", "liq", "no"). bulkRNA-Seq: Human telencephalic organoids; 5 replicates per condition of exogenous ECM supplementation (each sample is a single organoid).