Secretome analysis in CAF-dependent conditioned mediums with FaDu cells. Secretome analysis in CAF-dependent conditioned mediums with FaDu cells

We co-cultured primary cultured CAF (CAF-P), which promotes progression more actively, and CAF (CAF-D), which have less cancer-promoting properties, with OSCC cells, and analyzed the differential secretion through antibody microarray. Overall design: To evaluate the differential effect of the two CAF cell types on cisplatin efficacy in OSCC cells, we co-cultured FaDu spheroids (bottom well) with OSCC cells, CAF-P or CAF-D for 48 h in transwells. Antibody arrays were performed for each CM.