Supplementary Figure 11_contour plots for gh mAb binding

Supplementary Figure 11. Gating strategy andcontour plots to accompany binding data fromFigure 3b for each antibody (2 ug/mL) when incubatedwith fixed and permeabilized U373 astrocytomacell lines. (a) Representative gating strategyshowing U373-gH/gL cells stained with 5C3, apreviously characterized gH monoclonal, asa positive control for gH expression and binding.(b) A representative contour plot of AF647+ cellsfrom each test condition are shown for eachcell line. All conditions were performed intriplicate. Proportion of AF647+ cells are listedfor each plot.