Additional file 1: of HybriFree: a robust and rapid method for the development of monoclonal antibodies from different host species

Sequences of the primers used in VH and VL cDNA amplification for scFv-Fc construction. This file contains primer sequences used in the study for amplification of chicken, mouse or rabbit VH and VL domain cDNAs. Additional 5â nucleotides forming the overlapping region with the vector for CPEC cloning or the coding sequence of the flexible linker between the VH and VL domains are indicated. In addition, proportion of each primer in the cocktail is indicated. (XLSX 12 kb)