Short-time deep eutectic solvent pretreatment for enhanced enzymatic saccharification and lignin valorization

A comprehensive understanding of the structural and chemical nature of plant cell walls is important from both the perspectives of plant biotechnology and of commercial utilization. The Raman imaging technique is a preferred solution for its ability to offer spatial and spectral information simultaneously. However, the exact spectra of hemicellulose and cellulose are difficult to directly discern from each other due to the strong spectral overlap. In the present study, we report for the first time that the spectra of hemicellulose and cellulose in poplar cell wall Raman imaging can be discriminated by using multivariate analysis. The semi-quantitative concentrations of polysaccharides are identified based on the corresponding Raman images.