Primers for RT-qPCR amplification used in this study.
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aDesigned by an automated search in Primer Premier 5 software. Parameters for each primer pair: G+C content = 40–60%, annealing temperature between 58° and 62°C, and no false priming.bAmplicon size and specificity were checked using melt curve and 2% agarose gel electrophoresis (Figure S3).cEfficiency % = 10(−1/slope) −1. R2 values of all relative standard curves were more than 0.989.
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2015-12-02
