Prostaglandin modulated genes in L. major infected BMMs validated by qPCR.

Gene expression of prostaglandin pathway related genes in BALB/c Leishmania infected macrophages using qRT-PCR is analysed. Changes in mRNA levels are calculated using the 2−ΔΔCT method (ΔΔCt = ΔCt -ΔCt NI (t: point of the infection with P or KP) and ΔCt = Ct gene of interest- Ct of reference gene). Four reference genes were used for the qPCR normalization. The numbers presented for each time points are the average of the three biological replicates. The abbreviations NI, P and KP respectively refer to Non Infected macrophages, macrophages infected with live Parasite, macrophages infected with Killed Parasite.