Biotechnological production and purification of Borrelia burgdorferi sl B12 protein

Borrelia burgdorferi B12 protein could be used in the detection of specific immunoglobulins from patients' sera with Lyme disease that may be infected with different genospecies of B. burgdorferii sl. The goal of the study was to obtain the highest possible yield of protein expression in prokaryotic cells and purified protein preparations. This dataset contains data regarding the biotechnologial production and purification of a recombinant B. burgdorferii sl protein named "B12". Data includes the methodology, recombinant protein sequences and an image files with results of SDS-PAGE showing recombinant protein expression in E. coli cells and purified protein preparations. This research was funded by the National Science Centre, Poland, under the research project no. 2023/49/B/NZ6/02881