Effect of SNHG6 depletion on gene expression in radioresistant RTR61 cells

Head and Neck Squamous Cell Carcinoma (HNSCC) is a common cause of cancer death. Despite enormous technical advances in surgery and radiotherapy in the recent decade, survival of HNSCC patients has not markedly improved, with only 30% of patients diagnosed with advanced HNSCC that will survive for 5 years. This highlights the need to look into molecular processes leading to mechanisms of HNSCC radioresistance in HNSCC and identify novel radiosensitizers. Up-regulation of the SNHG6 micropeptide is associated with radioresistance of HNSCC cells. To assess the mechanism SNHG6-mediated radioresistance, we performed transcriptome analyses of RTR61 cells expressing either siScramble, or siSNHG6. We performed RNA-seq analysis of the cytoplasmic and total RNA isolated from RTR61 cells 72 hours after transfection with siScramble or siSNHG6. siPOOL SNHG6 or Scramble control, each containing a mixture of 30 different siRNAs (BioTech).