MAF Amplification licenses Estrogen Receptor a to Drive Breast Cancer Metastasis [ER ChIP-seq]

We performed genome-wide mapping of estrogen receptor (ER) binding sites in control and MAF-overexpressing MCF7 cells to assess the consequences of estrogen (E2) stimulation upon metastasic MAF expression. To this end, we cultured MCF7 cells in hormone-deprived (HD) medium for 72 h and then E2 or vehicle was added for 1h prior to chromatin immunoprecipitation (ChIP). Samples were generated in triplicate. We report that E2 induces extensive ER recruitment to chromatin and that ER-binding is gained and expanded upon MAF overexpression. Overall design: Examination of ER binding sites in control and MAF-overexpressing MCF7 cells before and after E2 stimulation.