Identifying a Novel Connection Between the Fungal Plasma Membrane and pH Sensing

To identify cellular processes similarly regulated by both Rim101 and Cdc50, we compared the transcriptomes of the rim101? and cdc50? strains against WT. We observed a partial overlap in the transcriptional perturbation shared by the mutant strains. Most notably, these data demonstrate a role for both Rim101 and Cdc50 in regulating the gene expression of membrane-associated proteins, including those involved in lipid bilayer composition and organization. Furthermore, this class of membrane-associated genes co-regulated by Rim101 and Cdc50 demonstrate similar expression changes in the two mutant strains in both direction and magnitude. Overall design: Gene expression analysis of WT and rim101? and cdc50? mutant cells generated in triplicate by RNA-Sequencing.