Effect of ROCK1 and ZIPK knockdown on myosin phosphorylation.
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https://figshare.com/articles/dataset/_Effect_of_ROCK1_and_ZIPK_knockdown_on_myosin_phosphorylation_/1322273
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UASMC were transfected with siRNAs targeting ROCK1 or ZIPK or with negative control siRNA as described in the Materials and Methods section. Cells were lysed in SDS-gel sample buffer and subjected to SDS-PAGE and western blotting with anti-2P-LC20 (antibody recognizing LC20 only when phosphorylated at Thr18 and Ser19). 2P-LC20 levels are expressed relative to GAPDH as loading control. Values indicate the mean ± SEM (n = 20). *,**significantly different from control (*p < 0.001; **p < 0.01). Representative western blots are shown in Fig. B in S1 File.
Effect of ROCK1 and ZIPK knockdown on myosin phosphorylation.
创建时间：
2015-02-27
