Additional FISH images of methanotrophic (MOX, MITC849 probe double-labelled with 594 dye) symbionts in 10 μm sections of H. (S.) methanophila sponge (Mictlan Knoll individual), epifluorescence microscpopy

We double-labelled MTC849 (5` – CGTTAGCTCCACCACTAAG – 3`) with Atto594 dye (Biomers, Ulm, Germany), and hybridized it with sponge tissue in 20% formamide buffer using standard protocol (Duperron <i>et al.</i>, 2008). Photomicrographs were acquired with Zeiss Axioplan 2 epifluorescence microscope (Zeiss, Jena, Germany), 40x or 100x lenses (see file name). Three channels were used - 594 (MITC849), DAPI and FITC(autofluoresence).