Genome-wide atlas of promoters expression reveals contribution of transcribed regulatory elements to genetic control of disuse-mediated atrophy of skeletal muscles. Genome-wide atlas of promoters expression reveals contribution of transcribed regulatory elements to genetic control of disuse-mediated atrophy of skeletal muscles

Here we report genome wide estimation of expression profiles at promoter level in Soleus and EDL muscles in rat hindlimbs under unloading conditions. Overall design: Samples were collected from rat hindlimbs at normal state, after unloading for 24 hours, 3 days, 7 days; recovery after 7 days unloading for 24 hours, 3 days, 7 days. Extracted total RNA were proceeded into cDNA and submitted to nAnTi-CAGE libraries preparation with subsequent sequencing on Illumina HiSeq2500 (SE, 50bp)