Thermodynamic cost of GpA-derived TM segments integration.

The predicted (ΔGpred) energetic cost in kcal/mol of inserting versions of the GpA TM spanning region estimated using the biological hydrophobicity scale [2], [5] are provided solely for the basis of comparison. Negative ΔGpred values are indicative of TM disposition, while positive values indicate non-TM disposition. Mutated residues at positions 85 and 89 are shown in bold.