Reverse transcriptase PCR (RT-PCR) analysis of piggyBac transposase expression.
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Total RNA was extracted from transgenic pupae that expressed only the ECFP marker, and from wild-type pupae reared at 25°C. A reaction without reverse transcriptase (-RT) was performed with cDNA synthesis as a negative control. Harmonia axyridis ribosomal protein 49 (Ha-rp49) was used as an internal control.
创建时间：
2016-02-23
