Expression data from white adipose tissue of adult mice with mutations in Regulator of sex-limitation genes

Regulator of sex-limitation (rsl) is a recessive mouse phenotype in which the otherwise male-specific sex-limited protein (Slp) gene is expressed in females. Positional cloning in rsl mice led to the identification of mutations in two neighboring KRAB zinc finger transcriptional repressors, Rsl1 and Rsl2, and BAC transgenic rescue experiments verified their ability to repress male-specific genes in the liver. Microarrays were used here to identify RSL-sensitive genes in mouse abdominal white adipose tissue. Experiment type: sex comparison and genetic modification Total RNA was extracted from abdominal white adipose tissue of adult male and female wild type and Rsl-null mice. Equivalent amounts of RNA were pooled from five mice per sex and genotype. Two pools per sex and genotype were hybridized to the Affymetrix Mouse Genome 430 2.0 PM Array Strip.性别限制调控因子（Regulator of sex-limitation，rsl）是一种隐性小鼠表型，该表型下原本仅雄性特异性表达的性别限制蛋白（sex-limited protein，Slp）基因会在雌性小鼠体内激活表达。对rsl小鼠进行位置克隆（positional cloning）后，研究人员鉴定出两个相邻的KRAB锌指转录抑制因子Rsl1与Rsl2发生突变；细菌人工染色体（bacterial artificial chromosome，BAC）转基因拯救实验证实，这两个因子可抑制肝脏中雄性特异性基因的表达。本研究使用微阵列（microarray）技术，筛选小鼠腹部白色脂肪组织中受RSL调控的敏感基因。实验类型：性别对比与基因修饰。从成年野生型与rsl敲除（Rsl-null）雌雄小鼠的腹部白色脂肪组织中提取总RNA；将每一种性别与基因型的5只小鼠的总RNA按等量混合，每种性别与基因型设置两个混合样本，随后将这些样本与Affymetrix Mouse Genome 430 2.0 PM基因芯片阵列条带进行杂交。