Genome-wide analysis of cleavage sites in budding yeast (Saccharomyces cerevisiae)

In previous studies, high-throughput approaches for genome-wide profiling of RNA cleavage sites were developed (e.g. Parallel analysis of RNA end (PARE), 5'P sequencing (5Pseq), Genome-wide mapping of uncapped transcripts (GMUCT)), However, there were some problems with experimental procedures (e.g. Many of the cleavage sites may be mapped in the 3' transcripts ; It was difficult to accurately identify the multiple cleavage sites in one mRNA). Thus, we performed truncated RNA end sequencing (TREseq) using S. cerevisiae (sigma 1278b strain) in exponential growth phase to determine the truncated 5' RNA ends (cleavage sites) and analyze the sequence feature around the cleavage sites.