Gene expression profiling associated with knockdown of RNF20 in human normal and malignant lung epithelial cell lines
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https://www.ncbi.nlm.nih.gov/sra/SRP091771
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资源简介：
The experiment was designed to display differential gene expression profiling in one lung epithelial cell BEAS2-B, and three lung cancer cell lines A549, H1299, H460 cells upon knockdown of RNF20, by using RNAseq technology. Overall design: RNF20 was first attenuated in duplicated normal lung epithelial cell BEAS2-B, and three lung cancer cell lines A549, H1299, H460 by siRNA-mediated knockdown. Total RNA was extracted from duplicated cancers cells transfected with two independent control siRNA (Ctrl1/2) and RNF20 specific siRNAs (RNF1/2) at 72h post-transfection for RNAseq analysis. Differentially expressed genes in RNF20-attenuated lung epithelial cells were identified in comparison to that in cells transfected with control siRNA.
创建时间：
2019-09-23
