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Impairment of Muscle Regeneration in Muscleblind-like 3 Isoform Knockout Mice

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We have performed an RNA-seq experiment to identify expression and alternative splicing differences between WT and Mbnl3 isoform knockout mice E15 forelimbs. We have also identified and characterized transcriptome wide Mbnl3-binding sites in C2C12 cells and E15 forelimbs. 6 total samples were analyzed: E15 forelimbs from 3 WT and 3 MBNL3 delta 2/deltaE2 female mice (15dpc). For HITS-CLIP, 3 samples each of C2C12 cells, WT E15 forelimbs and Mbnl3 deltaE2 forelimbs were analyzed for Mbnl3 binding site analysis.

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