Transcriptional Regulatory Induced Phenotype (TRIP) screen raw sequence data for Mycobacterium tuberculosis response to isoniazid

We developed Transcriptional Regulator Induced Phenotype (TRIP) screening, and applied it to find new mediators of drug susceptibility in M. tuberculosis (MTB). The TRIP screen exploits a library of 207 Transcription factor induction (TFI) strains, representing 97% of annotated MTB regulators. Each TFI strain is engineered for conditional induction of a single MTB TF and expression of its associated regulon. We chemically-trigger induction of the pooled library grown in absence and presence of the first-line anti-TB drug isoniazid, and we quantify the proportion of each TFI strain in the pool by next-generation sequencing to identify regulatory adaptations that alter fitness under these conditions.