Attenuation phenotype of the recombinant AH/AAca virus in chickens.
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aSix-week-old specific-pathogen free chickens were inoculated i.n. with 106 EID50 of virus in a 0.1-ml volume. Swabs were collected from the birds on day 3 and day 5 postinoculation and titrated in eggs. bSix-week-old white Leghorn chickens housed in high-efficiency particulate air-filtered isolators were inoculated i.v. with 0.2 ml of a 1∶10 dilution of bacterium-free allantoic fluid containing virus for intravenous pathogenicity index (IVPI) testing, based on recommendations of the Office International Des Epizooties.cOnly animals that showed disease signs but recovered by the end of the observation period were identified as sick birds.
创建时间：
2015-12-02
