Datasets for GTN materials on 1H-NMR-based metabolomic data processing

These data come from the Ph.D. of Unai Escribano-Vazquez (https://theses.hal.science/tel-02866073) and were generated in the lab of Claire Cherbuy (Micalis Institute, Jouy-en-Josas, France).

The intestinal microbiota is involved in the regulation of several metabolic pathways of the host, leading to important host-microbiota interaction axes such as those involved in metabolic signalling and immune/inflammatory responses. This microbial community has the enzymatic machinery necessary to metabolize nutriments coming from diet, and is a key factor of the host's energetic metabolism. Fat consumption has increased considerably in recent decades. This high consumption of fat is harmful for health. On a high fat diet, the intestinal microbiota is in a dysbiotic state.

The objective of this study was to investigate the impact of a bacterial species, E. coli, which increases during fat consumption, on the metabolomic trajectory of mono-associated mice fed a standard and high fat diet. Two strains of E. coli were used in our study: the CEC15 strain we previously isolated from freshly pooled faecal samples of 15-day-old suckling rodents and the Nissle 1917 strain, as a representative member of the B2 E. coli phylogroup that has shown to increase in the human gut microbiote during the last decades.

In this tutorial, we used samples collected on male C57b mice with Germ Free+Nissle1917 or Conventionally microbiota, who underwent a High fat diet. We performed 1-H NMR analysis on this samples.