Transcriptional Response of Propionibacterium freudenreichii DSM 20271 to Riboflavin and Nicotinamide Supplementation in Malt Extract Medium

Vitamin B12 (cobalamin) biosynthesis in Propionibacterium freudenreichii relies on the availability of specific precursors, including 5,6-dimethylbenzimidazole (DMBI), the lower ligand of cobalamin. Riboflavin (RF) serves as a precursor for DMBI synthesis, while nicotinamide (NAM) has been reported to enhance this metabolic pathway. The goal of this study was to investigate the transcriptional response of P. freudenreichii subsp. freudenreichii DSM 20271 to RF and NAM supplementation in a barley malt extract-based model medium