Changes of intestinal microbiota in ovariectomized rats in 3 months

The purpose of this study was to explore the effect of gut microbiota on PMOP by observing the changes in the levels of estradiol, bone density, and gut microbiota diversity in rats after 3 months of OVX surgery. We randomly divided 60 female SD rats into four groups: baseline group (6 rats), sham-operated group (18 rats), model group (18 rats), and estrogen-treated group (18 rats). The ovariectomy model of postmenopausal osteoporosis was established by performing bilateral ovariectomy. After surgery, 6 rats from each group were randomly sacrificed every 30 days and assessed. After 90 days, all rats were sacrificed and their body weight, bone mineral density (BMD), tissue mineral density (TMD), trabecular bone parameters, femoral bone morphology, hormone levels, and gut microbiota diversity were assessed.