Interferon Alpha Induces Multiple Cellular Proteins That Coordinately Suppress Hepadnaviral Covalently Closed Circular DNA Transcription. Interferon Alpha Induces Multiple Cellular Proteins That Coordinately Suppress Hepadnaviral Covalently Closed Circular DNA Transcription

To identify the cellular genes that mediate IFN-α suppression of cccDNA transcription, the total intracellular RNA were extracted from untreated or recombinant chicken IFN-α (rChIFN-α)-treated cells, and mRNA sequencing (mRNA-Seq) assays were performed in triplicate. And we found that treatment of dstet5 cells with 10 ng/ml of rChIFN-α for 6 h altered the expression of more than 100 cellular genes by more than 2 folds. Overall design: mRNA sequencing (mRNA-Seq) assays were performed in triplicate for untreated or rChIFN-α-treated dstet5 cells