Kamminga2017 - Metabolic model of Mycoplasma hyopneumoniae growth

Kamminga2017 - Metabolic model of Mycoplasma hyopneumoniae growth

This model is described in the article:

Metabolic modeling of energy
balances in Mycoplasma hyopneumoniae shows that pyruvate
addition increases growth rate.

Kamminga T, Slagman SJ, Bijlsma JJE,
Martins Dos Santos VAP, Suarez-Diez M, Schaap PJ.
Biotechnol. Bioeng. 2017 Jun; :
Abstract:

Mycoplasma hyopneumoniae is cultured on large-scale to
produce antigen for inactivated whole-cell vaccines against
respiratory disease in pigs. However, the fastidious nutrient
requirements of this minimal bacterium and the low growth rate
make it challenging to reach sufficient biomass yield for
antigen production. In this study, we sequenced the genome of
M. hyopneumoniae strain 11 and constructed a high quality
constraint-based genome-scale metabolic model of 284 chemical
reactions and 298 metabolites. We validated the model with
time-series data of duplicate fermentation cultures to aim for
an integrated model describing the dynamic profiles measured in
fermentations. The model predicted that 84% of cellular energy
in a standard M. hyopneumoniae cultivation was used for
non-growth associated maintenance and only 16% of cellular
energy was used for growth and growth associated maintenance.
Following a cycle of model-driven experimentation in dedicated
fermentation experiments, we were able to increase the fraction
of cellular energy used for growth through pyruvate addition to
the medium. This increase in turn led to an increase in growth
rate and a 2.3 times increase in the total biomass
concentration reached after 3-4 days of fermentation, enhancing
the productivity of the overall process. The model presented
provides a solid basis to understand and further improve M.
hyopneumoniae fermentation processes. Biotechnol. Bioeng.
2017;9999: 1-9. © 2017 Wiley Periodicals, Inc.

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