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Four thermal protocols used with different PCR reaction and chip designs.

NIAID Data Ecosystem2026-03-08 收录

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https://figshare.com/articles/dataset/_Four_thermal_protocols_used_with_different_PCR_reaction_and_chip_designs_/1034205 数据链接链接失效反馈

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*Initial cycles done only in presence of long primers.

应用场景：

创建时间：

2014-05-22

基因组学

PCR技术

Table S3. Primer list. The sequence surrounding NNN-islands in the CRBO_1381 assembly scaffold was entered into Primer3 with default settings (GC clamp = 1) to find suitable primersets for PCR product

Mendeley Data2024-06-27 更新70

Primers used and PCR amplicons generated in this study.

分子生物学

PCR技术

*The restriction endonuclease (R.E.) site included in each primer is underlined and the corresponding R.E. indicated in the adjacent column. #The amplicons generated with a given set of PCR primers ar

NIAID Data Ecosystem60

C t value for PCR reagent stored at 37°C for different days.

PCR技术

分子生物学

aCt: threshold cycle;values<35 are considered to be positive. Each Ct value is the mean result of the three values obtained each time point. bCV: coefficients of variations.

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Characteristics of the primers used for SYBR green Q-PCR assays.

分子生物学

PCR技术

*Letters F and R after the primer name indicate forward and reverse orientation, respectively. †Theoretical melting temperature (Tm) calculated using the MWG Oligo Property Scan (MOPS).

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Effectiveness of primer pairs to detect HBV DNA in HBsAg (-)/anti-HBc (+) patients from Puebla Mexico by nested PCR.

HBV乙型肝炎病毒

PCR技术

Effectiveness of primer pairs to detect HBV DNA in HBsAg (-)/anti-HBc (+) patients from Puebla Mexico by nested PCR.

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