Geminin regulates self-renewal and fate commitment decisions in fetal hematopoietic stem cells.

Conditional deletion of Geminin from the entire hematopoietic compartment using Vav1:iCre mice led to defective hematopoiesis/dyserythropoiesis in E15.5 mouse embryos. The present data set includes data from lineage-negative cells isolated from homogenized livers that were dissected from E15.5.dpc embryos. The two conditions compared were wild-type versus Geminin-KO Lin- cells. The cells were collected from littermates. Six samples were analyzed: three samples from each wild-type and Geminin-KO Lin- cells. For our analyses, we set cutoff values FC>1.5 and p-value <0.05. The analysis was carried out using the genotype criterion, but non-averaged for the three biological replicates.利用Vav1:iCre小鼠对全造血区室中的Geminin进行条件性敲除，可导致胚胎发育第15.5天（E15.5）的小鼠胚胎出现造血功能缺陷与红系生成异常。本数据集包含从E15.5天胚胎解剖获取的匀质化肝脏中分离得到的谱系阴性细胞（Lin-，lineage-negative）相关数据。本次对比的两组样本为野生型（wild-type）与Geminin基因敲除（Geminin-KO）的Lin-细胞。所有细胞均取自同窝胚胎。共分析6份样本：野生型与Geminin-KO Lin-细胞各3份。本分析设定的筛选阈值为折叠变化（FC，Fold Change）>1.5且P值<0.05。本次分析以基因型作为分组依据，且未对3份生物学重复样本进行均值化处理。