Pseudotime reconstruction of cytokinesis - examples

These two dataset represent a subset of the raw data that was acquired for pseudotime reconstruction of the intercellular bridge development in 6 cytokinetic steps. The data are fluorescence images of expanded NRK49F cells, undergoing cytokinesis. The images were acquired as z-stacks with 1 µm spacing and a x/y-pixel size of 90 nm on a spinning disk confocal microscope. Septin2-GFP, tubulin, a variable target and DAPI are visualized in 4 channels. The dataset is related to Zach Marin's 'Cytokinesis Pseudotime Analysis' package https://github.com/zacsimile/cytokinesis-pseudotime-analysis In order to test run the code, two different targets (MKLP1 and actin) are provided in /...example and /...example_2. Each target includes images of 6 differet stages (RC, CS, RS, SM, BA, A). The included xlsx-files provide the necessary information (length, X, Y) for aligning the intercellular bridges. A more detailed instruction for the use of this datset can be found on the Github page for 'Cytokinesis Pseudotime Analysis'. This version includes the targets.yaml file, which simplifies the integration of the example data with the script looped_images_over_pseudotime copy.ipynb.