Tracking polyhydroxyalkanoate biosynthesis in thermophilic microorganisms

"Tracking PHA biosynthesis in thermophiles: supplementary dataset"

This dataset accompanies the publication "Tracking PHA biosynthesis in thermophiles" and contains comprehensive data used in the analysis of polyhydroxyalkanoate (PHA) synthase (PhaC) sequences across archaeal and bacterial thermophiles. The provided data includes sequence alignments, classifications, and detailed lists of putative and verified PhaC sequences identified through bioinformatic analyses.

Contents:

Supplementary File S1: A categorized list of archaeal and bacterial genera/classes based on optimal growth temperature.

Supplementary Table S1: Detailed inventory of microorganisms from which PhaC sequences were utilized in bioinformatic analyses.

Supplementary Figure S1: Multiple sequence alignment visualization of 16 well-characterized or automatically annotated reference PhaC sequences.

Supplementary Figure S2: Matrix illustrating pairwise percent identities among aligned PhaC protein sequences.

Supplementary File S2: Identification and annotation of putative PhaC sequences from bacterial moderate thermophiles, highlighting discrepancies in lipase-like box motif alignments.

Supplementary File S3 (Zenodo): Complete multiple sequence alignment of 366 putative bacterial moderate thermophilic PhaC sequences, including the 16 reference sequences.

Supplementary Table S2: Curated and filtered dataset of bacterial moderate thermophilic PhaC sequences selected for further analysis.

Supplementary File S4: Identification and annotation of putative PhaC sequences from archaeal moderate thermophiles, including details on lipase-like box motif alignments.

Supplementary File S5 (Zenodo): Complete multiple sequence alignment of 256 putative archaeal moderate thermophilic PhaC sequences alongside the 16 reference sequences.

Supplementary Table S3: Curated and filtered dataset of archaeal moderate thermophilic PhaC sequences selected for further analysis.

Supplementary File S6: Identification and annotation of putative PhaC sequences from bacterial extreme thermophiles, highlighting discrepancies in lipase-like box motif alignments.

Supplementary File S7 (Zenodo): Complete multiple sequence alignment of 164 putative bacterial extreme thermophilic PhaC sequences, including the 16 reference sequences.

Supplementary File S8: Identification and annotation of putative PhaC sequences from archaeal extreme thermophiles, highlighting discrepancies in lipase-like box motif alignments.

Supplementary File S9 (Zenodo): Complete multiple sequence alignment of 26 putative archaeal extreme thermophilic PhaC sequences alongside the 16 reference sequences.

Supplementary Table S4: Curated and filtered dataset of archaeal extreme thermophilic PhaC sequences selected for further analysis.

Supplementary Figures S3-S5: Analytical results of PHA characterization, including gas chromatography-mass spectrometry (GC-MS), differential scanning calorimetry (DSC), and proton nuclear magnetic resonance (¹H-NMR).

DOI: 10.5281/zenodo.15221919