Project Section: Searching for Mutations Leading to Isoniazid Resistance in TB (HackBio)

Introduction

Tuberculosis (TB) remains a global health concern, and the emergence of drug-resistant strains poses a significant challenge to effective treatment. Isoniazid is a key first-line drug used in the treatment of TB, and resistance to this drug can compromise the success of therapy.

This project aims to identify and characterise genetic mutations associated with isoniazid resistance in Mycobacterium tuberculosis, the causative agent of TB.

Datasets: Here

Task:

Given the datasets above, perform the complete variant calling and annotation pipeline to specifically identify the following known mutations in Mycobacterium tuberculosis (against Isoniazid).

Provide pretty IGV visualisation of mutations in katG sequences (Position: 2156111 - 2153889) across all the samples. N/B: katG mutations are prevalent in isoniazid resistance.

Also, explore associated biological pathways to understand the molecular mechanisms of isoniazid resistance.

Expected Output:

Kindly submit a comprehensive project report, including methods, results, and interpretations.