Mapping by multifragment cloning in vivo.
收藏PubMed Central1995-12-05 更新2026-05-02 收录
下载链接：
https://pmc.ncbi.nlm.nih.gov/articles/PMC40470/
下载链接
链接失效反馈官方服务：
资源简介：
An efficient method for mapping mutations is described in which hybrid genes, derived partly from mutant and partly from wild-type DNA, are obtained in vivo by homologous recombination of multiple fragments. The recombinants are formed in a strain in which their phenotypes are immediately apparent. This method was developed to identify changes that disrupt protein-protein interactions demonstrable by the two-hybrid system in yeast. However, it can be extended to any system where recombination is possible, provided an assay is available to distinguish between mutant and wild-type phenotypes. IMAGES:
提供机构：
National Academy of Sciences
创建时间：
1995-12-05
