Citrus sinensis challenged with LotP

Infiltrating purified LotP protein in healthy citrus leaf induces chlorosis and provoke plant stress. Further proteomic analysis of the affected tissue using TMT11MS/MS revealed a significant abundance of plant proteins associated with stabilizing and processing mRNA transcripts and miRNAs. Interestingly, a subset of transcription factors directly connected to pathways coordinating the innate plant defense were also more abundant in the damage tissue two days post infiltration. Inhibiting the formation of LotP multimers via direct mutation of critical residues of the N terminal domain significantly decreases the chlorotic response in infiltrated leaf as well as the intensity of the associated proteome reaction.