No detectable alloreactive transcriptional responses under standard sample preparation conditions during donor-multiplexed single-cell RNA sequencing of peripheral blood mononuclear cells

We employed an experimental design where PBMCs from a single donor was sequenced with or without mixing with PBMCs from other healthy unrelated donors in order to determine whether PBMCs engage in an allogeneic response under standard scRNA-seq sample preparation conditions (e.g., 30 minute co-incubation at 4C) Overall design: Single-cell gene expression profiles of PBMCs from unrelated healthy donors tagged with MULTI-seq LMOs and/or antibody-DNA conjugates from the BD Single-Cell Multiplexing Kit (SCMK)