Additional file 9: of First gene-ontology enrichment analysis based on bacterial coregenome variants: insights into adaptations of Salmonella serovars to mammalian- and avian-hosts

Candidates of PAI-like region overlapping genomic islands (cPAIs) impacted recombination events identified in Salmonella enterica subsp. enterica serovars Dublin, Enteritidis, Pullorum and Gallinarum. The variants were identified by the workflow ‘VARCall’ against the reference genome S. Enteritidis (strain P125109, accession NC_011294.1). The produced pseudogenomes (4,685,848 bp) were inferred with RAxML based on a bootstrap analysis and search for best-scoring Maximum Likelihood tree with General Time-Reversible model of substitution and the secondary structure 16-state model. The pseudogenomes and the RAxML inference were used to perform detection of recombination events based on default gamma priors of ClonalFrameML. The recombination events with sizes higher than 400 bp are presented. Pathogenicity Island Database from KonKuk University (Seoul, South Korea) were used to detect Salmonella Pathogenic Islands (SPIs) SPI-1 (2890501–2,934,879), SPI-2 (1727425–1,769,273), SPI-4 (4333507–4,361,514), SPI-5 (1053174–1,074,167), SPI-6 (299796–330,890), SPI-11 (1904313–1,912,607), SPI-12 (2328077–2,347,757) and PAI III 536 (2801306–2,810,695) of the reference genome S. Enteritidis (strain P125109, accession NC_011294.1). (XLSX 19 kb)