Analysis of the role of Mcu in the regenerating mouse liver after partial hepatectomy. Analysis of the role of Mcu in the regenerating mouse liver after partial hepatectomy

In this study, we analyzed global liver gene expression in MCU knock-down (KD) mice. MCUloxp/loxp male mice were treated with an AAV8-Cre under the control of a hepatocyte specific promoter (TBG) to generate liver-specific MCU KD mice. AAV8-TBG-Null treated littermates were used as controls. Knockdown was verified 3 weeks after injection by protein and mRNA (80%). 5 weeks after injection mice were subjected to 70% partial hepatectomy and liver semples were collected 30h after the sugery. Mouse Clariom S (Affymetrix, Santa Clara, CA) arrays were used to obtain global gene expression data from pooled liver samples (pools of 4 biological replicates per array). Overall design: Gene expression was profiled in the regenerating livers of wild type and in liver-specific MCU knockdown mice at t=0 and 30h after 70% partial hepatectomy; pools of 4 biological replicates per genotype.