Interaction of non-lamellar RNA-loaded lipid nanosystems with synthetic lipid vesicles that mimic the endosomal membrane: a time-resolved st

Nowadays, intensive research is devoted to the development of efficient RNA vectors, able to efficiently complex the genetic material and deliver it to its biological target. In this proposal we would like to investigate the structure and potential efficacy of newly developed lipid-based formulations of non-lamellar structure, containing biocompatible cationic and/or ionizable lipids, for the delivery of RNA-based therapeutics. We will investigate (i) the structure of the new formulations in different experimental conditions, via static SAXS experiments (ii) the interaction mechanism/kinetics of the different formulations with synthetic lipid vesicles mimicking endosomal membranes under different pH conditions, via stopped-flow SAXS. This research seeks to advance our understanding on how these lipid particles interact with lipid vesicles and will contribute to the development of evermore effective RNA delivery systems.