Microscopy images from: A cadherin-integrin-ECM code for presomitic mesoderm fluidity

Animal tissues exist within a continuum of fluid to solid states, and transitions between states are important for embryonic development, wound healing and cancer metastasis. Fluid to solid transitions are governed by the ratio of adhesive energy to kinetic energy. Here, we find that presomitic mesoderm solidification is driven by an intrinsic decline in cell speed along with an increase in adhesion mediated by Cadherin 2 in parallel with Fibronectin and its receptor Integrin α5. A computational model of cell-cell adhesion in the central tissue mesenchyme and cell-ECM adhesion on the tissue surface explains the observed phenotypes. Further, we identify negative feedback within the ECM as Fibronectin supports the formation of a separate layer of Fibrillin 2b matrix that inhibits solidification. These data reveal a tissue fluidity code in which solidification is promoted by cadherins in parallel with Integrin α5 and Fibronectin, whereas negative feedback through Fibrillin 2b promotes flui..., , # Microscopy images from: A cadherin-integrin-ECM code for presomitic mesoderm fluidity

Dataset DOI: [10.5061/dryad.wstqjq30g](https://doi.org/10.5061/dryad.wstqjq30g)

## Description of the data and file structure

This dataset contains microscopy images of the zebrafish PSM obtained for the purpose of calculating cell shape and packing fraction (Figure 2), cell velocity (Figure 3), and ECM structure (Figure 5) in A cadherin-integrin–ECM code for presomitic mesoderm fluidity. 

### Files and variables

#### File: cellposemodels.zip

**Description:** Custom trained Cellpose 2.0 models for segmenting cells in coronal and transverse orientations using membrane-RFP and secreted-GFP. See Figure 2C and S4.

Two models: one for XY and one for XZ orientation for use with cellpose 2.0

XY model: itgcdh_xy_gray

Trained on images of MZ-itga5-; cdh2- expressing memRFP, secreted GFP,

Segmentation done in grayscale ie channels=[0, 0]

Presumably cellposes's grayscale conversion is 1/2 red intensi...,