Expression analysis of tumor-associated macrophages from primary tumor and metastatic lung

In tumor microenvironment, tumor-associated macrophages (TAMs) have been characterized as M1-like or M2-like phenotype. In this study, we investigated the characteristics and functional roles of different TAMs on cancer metastasis. We isolated TAMs from primary tumor and metastatic lung and performed microarrays to identify the gene expression in distinct TAMs populations. TAMs were sorted by CD11B+ and F4/80+ from tumor-bearing mice. Bone marrow-derived macrophages were differentiated from the bone marrow of health mice and sorted by CD11b+ and F4/80+.