Repeatable randomness, invariant properties, and the design of biological signatures of identity

What makes a perfect signature? Optimal signatures should be consistent within individuals and distinctive between individuals. In defence against avian brood parasitism, some host species have evolved ‘signatures’ of identity on their eggs, comprising inter-individual variation in colour and pattern. Tawny-flanked prinia (Prinia subflava) egg signatures facilitate recognition and rejection of parasitic cuckoo finch (Anomalospiza imberbis) eggs. Here, we show that consistency and distinctiveness of pattern are negatively correlated in prinia eggs, perhaps because non-random, repeatable pattern generation mechanisms increase consistency but limit distinctiveness. We hypothesise that pattern properties which are repeatable within individuals but random between individuals (‘invariant properties’) allow hosts to circumvent this trade-off. To find invariant properties, we develop a method to quantify entire egg phenotypes from images taken from different perspectives. We find that marking s..., Dataset consists of pattern metrics extracted from egg images, and egg rejection data, from field experiments conducted from 2018-2020. ,