Transcriptional profiling of indica rice cultivar FR13A under salt stress condition.

A submergence tolerant indica rice cultivar FR13A, was also reported to withstand salt stress and proven in our experiments. The mechanism of tolerance is yet to be studied by forward genetics approach. However, it is known that salt stress tolerance is governed by several QTLs and not by a single gene. To understand the mechanism of such a complex mechanism of salt tolerance we selected, two indica rice genotypes namely, I) FR13A, a tolerant indica variety and ii) IR24, a susceptible genotype for this study. We used the 22K rice Oligoarray from Agilent technologies to study the transcript profile in the leaves of the two contrasting rice genotypes under constitutive and salt stress conditions at seedling stage. Keywords: Mechanism of salt tolerance Overall design: We used Agilent rice gene chips (G4138A) to investigate the transcript level changes in rice plant tissues during salt stress treatment. We used two contrasting rice genotypes (FR13A tolerant and IR24 susceptible) differing in salt stress response. Plants were grown in growth chambers and treated with 150 mM salt concentration at 14th DAS. Sampling was done in both constitutive and treated plants at 3 time points. Two replications of microarray experiments were carried out by hybridizing the RNA from tolerant samples against the susceptible lines on the same slide.

一种耐淹水稻品种FR13A，亦被报道能抵御盐胁迫，并在本实验中得到验证。耐受性的机制尚需通过正向遗传学方法进行研究。然而，已知盐胁迫耐受性受多个数量性状基因座（QTLs）的控制，而非单一基因。为了揭示这一复杂耐受机制，我们选取了两种印度水稻基因型进行研究，即：I) 具有耐受性的印度水稻品种FR13A，ii) 对该胁迫敏感的基因型IR24。我们采用Agilent技术提供的22K水稻寡核苷酸阵列，研究在幼苗阶段，两种对比鲜明的水稻基因型在恒定和盐胁迫条件下的转录组特征。关键词：盐胁迫耐受机制总体设计：我们利用Agilent水稻基因芯片（G4138A）研究在盐胁迫处理过程中水稻组织转录水平的变化。我们选取了两种在盐胁迫响应上存在差异的对比水稻基因型（FR13A耐受型和IR24敏感型）。植物在生长箱中培养，并在第十四天出现盐胁迫（DAS）时用150 mM的盐浓度进行处理。在恒定和胁迫处理的植物中，于三个时间点进行采样。通过将耐受样本的RNA与敏感系在同一玻片上进行杂交，进行了两次微阵列实验的重复。