Single cell RNA sequencing of adult regenerating mouse digit tips

Innate regeneration following digit tip amputation is one of the few examples of epimorphic regeneration in mammals. Digit tip regeneration is mediated by the blastema, the same structure invoked during limb regeneration in some lower vertebrates. By genetic lineage analyses in mice, the digit tip blastema has been defined as a population of heterogeneous, lineage restructed progenitor cells. These previous studies, however, do not comprehensively evaluate blastema heterogeneity or address lineage restruction of closely related cell types. Here we report single cell RNA sequencing of over 38,000 cells from mouse digit tip blastemas and unamputated control digit tips and generate an atlas of the cell types participating in digit tip regeneration. Overall design: Adult wildtype FVB/NJ mice used to generate 5 distinct datasets throughout digit tip regeneration. The five stages are: unamputated (control), 11 days post-amputation, 12 days post-amputation, 14 days post-amputation, and 17 days post-amputation.