PFOS negatively impacts prey capture in larval zebrafish

Per- and polyfluoroalkyl substances (PFAS) are widely used in many industrial and domestic applications. The wide range use of PFAS has resulted in unintentional human exposures and bioaccumulation in blood and other organs. Perfluorooctanesulfonate (PFOS) is among the most prevalent PFAS in the environment and has been postulated to affect brain functions in exposed organisms. However, the impacts of PFOS on early neural development have not been well-described. Here, we used zebrafish larvae to assess the effects of PFOS on two fundamental complex behaviors, prey capture and learning. Zebrafish exposed to PFOS concentrations ranging from 2 – 20 µM for differing 48-hour periods were viable through early larval stages. In addition, PFOS uptake was unaffected by the presence of a chorion. We employed two different experimental paradigms; we first assessed the impacts of increasing organismal PFOS bioaccumulation on prey capture and learning, and second, we probed stage-specific sensitivi..., Zebrafish embryos and larvae were exposed to PFOS in multiple different treatment and feeding groups. Subsequent behavior testing was done blinded to both the treatment and feeding group. Following behavior testing, samples were collected for LC-MS/MS analysis, which was also done blind to treatment and feeding group. After the collection of the LC-MS/MS data, the PFOS measurements and behavior analysis were linked to their respective feeding and treatment groups (By the combination of their Group # and Sample #). Analysis of the aggregate data (Tukey HSD and multiple linear regressions) were then performed on each dataset., , # PFOS negatively impacts prey capture in larval zebrafish

Included in uploaded files are the LC-MS PFOS measurements and all collected paramecia prey capture data for all experiments.

## Description of the data and file structure

This paper combines two main experimental paradigms: one tested the developmental time-period of PFOS exposure and the other tested the concentration of PFOS exposure. Our data is uploaded as two separate Excel files for each experiment. Each excel file contains 3 tabs:

1. Behavior Key - This is the Key that we used to unblind our results after analysis. It identifies which groups were linked to which treatment groups.
2. PFOS Measurements - This is the PFOS measurement values from the LC-MS/MS measurements of the zebrafish larvae following behavior.
3. Behavior Measurements - This includes the feeding group for each sampled fish, the number of starting paramecia in their experiment dish, the number of ending paramecia after feeding, as well as a calc...