TXNIP loss expands Myc transcriptome

To examine the generality of our finding where the gene expression profile of TXNIP knockout in MDA-MB-231 cells resembles of that Myc overexpression transcriptional program, our lab has generated TXNIP null HCC70 (HCC70:TKO) and MB135 (MB135:TKO) cells. We characterized the gene exrpession programs of these cells by RNA-seq. Overall design: TXNIP was knocked out in HCC70 and MB135 cells using CRISPR-Cas 9 method. Parental HCC70 or MB135 cells and TXNIP knockout HCC70 (HCC70:TKO) or MB135 (MB135:TKO) cells were used to perform RNA sequencing. Parental MB135 and MB135:TKO cells were differentiated for 5 days before RNA was harvested for RNA sequencing.