RNA-sequencing of NALT from mouse after intranasal immunization with B. abortus rMDH loaded chitosan nanoparticles (CNs)

Brucellosis is a serious zoonotic disease caused by the genus Brucella, a group of Gram-negative and facultative intracellular bacteria. At present, although live attenuated vaccine such as Rev.1, S19 and RB51 have been widely used for bovine brucellosis, these vaccines have some drawbacks, and safe and effective alternatives have been demanded. Previously, our group showed that several Brucella proteins malate dehydrogenase (rMDH) are effective in eliciting IgG responses in murine model following intraperitoneal injection. The Brucella infections are usually transmitted through mucosal membrane via oral or aerosol exposure. Therefore, induction of mucosal immunity is promising contribute in development of vaccine. In the study, rMDH was loaded with chitosan nanoparticles (CNs) which is mucoadhesive, biocompatible, nontoxic, and immune-enhancing adjuvant to induce both systemic and mucosal immunity. Then, in order to evaluate immunogenicity of the antigen in BALB/C mouse, total RNA from nasal-associated lymphoid tissues at 1 h, 6 h, 12 h after intranasal immunization was analyzed using RNA-seq. mRNA profiles of NALT from mice at 1 hour, 6 hours, and 12 hours after immunization with CNs - rMDH. PBS *h = NALT from mice at *hours after immunization with PBS as control 1 CNs *h = NALT from mice at *hours after immunization with chitosan nanoparticles (CNs) only as control 2 TF *h = NALT from mice at *hours after immunization with rTrigger Factor (recombinant protein vector) loaded CNs as control 3 MDH *h =NALT from mice at *hours after immunization with rMDH loaded CNs as test group.