Transcriptome analysis of C2C12 cells (WT and MYOD KO) overexpressing MUNC: proliferating and differentiating conditions.. Mus musculus

To check global changes of genes expression correlating with induction of MUNC, we stably overexpressed MUNC in WT cells and in MYOD KO cells. We compared transcriptomes of control cells and cells overexpressing MUNC and distinguished genes that are differentially expressed in different conditions Overall design: C2C12 WT cells were stably transfected with control vector (EV) and vector coding for MUNC spliced (ovMUNC). MYOD-/- cells were generated by CRISPR-Cas9 method, and after single cell screeening and deletion confirmation, MYOD-/- cells were transfected with control vector (EV) or the vector coding for MUNC spliced (ovMUNC). RNA samples were isolated from proliferating or differentiating (DM3) cells. 1 ug of RNA per sample was enriched for polA tailed mRNA molecules and RNA-Seq libraries were made. Pooled libraries were sequenced using paired-end protocol on Illumina platform