Identification of proteins that presented a significant increase in their expression content.

a) Spots match ID number obtained from ImageMaster Platinum;
b) Name of the identified protein;
c) Uniprot identification code;
d) Experimentally predicted and expected isoelectric point (pI);
e) Experimentally predicted and expected molecular weight (Mr, in kDa);
f) Number of identified peptides by MS;
g) Percentage of the protein sequence covered by identified peptides;
h) Normalized data from R0 represented by mean values of each condition divided by R30 value;
i) Fold represents the maximum spot intensity mean value of the conditions divided by the smallest value;
j) One-way ANOVA (P<0.01) obtained from spot analysis;
k) Biological functions according to NCBI, UniProt, and Gene Ontology databases;
l) Biological activity and/or immunological application described in other studies: [53] Joshi et al., 1996; [54] Tielens et al., 2010; [55] Soto et al., 1996; [56] Lackovic et al., 2010; [57] Scher et al., 2012; [58] Sánchez-Cañete et al., 2009; [59] Peris et al., 1994; [60] Steiner et al., 2007; [61] Jaramillo et al., 2011; [62] Duclert-Savatier et al., 2009; [63] Celeste et al., 2004; [64] Liu et al., 2011; [65] Leblanc et al., 1998; [66] Priest et al., 1996; [67] Bakker-Grunwald, 1992; [68] Banerjee et al., 2006; [69] Casanova et al., 2008; [70] Jensen et al., 2001. The proteins were identified through the data included in the NCBI database (dated June 2012) for Leishmania spp.