Identification of neural oscillations and epileptiform changes in human brain organoids

In our study, we define neural network activities in human cortex+ganglionic eminence (GE) fusion orgaonids using a combination of calcium incator imaging and electrophysiology. We futher define abnormal network activities using fusion organoids generated from isogenic induced pluripotent stem cells containing or lacking MECP2 function derived from Rett syndrome patients. Single cell sequencing (10X genomics) was used to characterize the compositon of the fusion organoids used in our studies, and to define differentially expressed genes associated with MECP2 mutation. Overall design: Comparison of pools of organoids generated from isogenic control (WT) or MECP2 mutant (705delG/ E235fs; Mut) iPSC derived from a Rett syndrome patient, collected at 3 time points (Days 56, 70, and 100). Day 56 pools each contain 3 unfused Cortex and 3 unfused Ganglionic eminence organoids. Day 70 and 100 pools contain 3 Cortex+Gangiolnic eminence fusion organoids.