Single cell transcriptomic profiling reveals that CD9 is a negative marker of glucose-responsive pancreatic beta-like cells derived from human pluripotent stem cells. Homo sapiens

We generated an efficient protocol for differentiating islet-like organoids from human embryonic stem cells (hESC-islets) with beta-like cells being the predominant endocrine cell type. We performed single cell transcriptomic profiling with hESC-islets and found that CD9 is a negative cell surface marker for beta-like cells as most INS+ cells are CD9-.